(Ultra) High Performance Liquid Chromatography (HPLC/UHPLC)
The high-performance liquid chromatography (HPLC) technique involves separating the substances in a sample in a chromatography column according to their affinities to a liquid phase (eluent) and a solid phase (column material).
Using flow rates of 0.5 to 2 mL/min, pressures of up to 300 bar with UHPLC of even up to 1,300 bar, are built up in state-of-the-art systems and high-resolution columns.
The separated substances are then detected, identified according to their time of flow and quantified. During this process, a variety of detector types which measure absorption, fluorescence or electrochemical responses are used.
Our most important Routine determinations with UHPLC
| CD transferrin | S | β-carotene | S/P | |||
| glycated haemoglobin (HbA1c) | E | Vitamin A/E | S/P | |||
| Homovanillylmandelic acid | CU | Vitamin B1 | E | |||
| 5-hydroxyindoleacetic acid | CU | Vitamin B2 | E | |||
| Catecholamines | CU | Vitamin B6 | E | |||
| Metanephrines | CU | Vitamin B6 | S/P | |||
| Porphyrins | CU | Vitamin C | HPG | |||
| Serotonin | SG | Coenzyme Q10 | S/P | |||
| Vanillylmandelic acid | SU |
Key material for analysis: S=Serum, SG=Serum frozen, P=Plasma, HPG=Heparin plasma frozen, U=Urine, CU = Collected urine, E=EDTA blood
LC-MS/MS was used instead for all medications determined by chromatography.
Your contact persons
DrRolf Dietiker
Head COE Data & Processes
Special Analytics, Drug Analytics
dipl. pharm.Carmen Bürki
Head Special Analytics
FAMH Clinical Chemistry, Haematology