(Ultra) High Performance Liquid Chromatography (HPLC/UHPLC)

The high-performance liquid chromatography (HPLC) technique involves separating the substances in a sample in a chromatography column according to their affinities to a liquid phase (eluent) and a solid phase (column material).

Using flow rates of 0.5 to 2 mL/min, pressures of up to 300 bar with UHPLC of even up to 1,300 bar, are built up in state-of-the-art systems and high-resolution columns.

The separated substances are then detected, identified according to their time of flow and quantified. During this process, a variety of detector types which measure absorption, fluorescence or electrochemical responses are used.

 

 

Our most important Routine determinations with UHPLC

CD transferrin S β-carotene S/P
glycated haemoglobin (HbA1c) E Vitamin A/E S/P
Homovanillylmandelic acid CU Vitamin B1 E
5-hydroxyindoleacetic acid CU Vitamin B2 E
Catecholamines CU Vitamin B6 E
Metanephrines CU Vitamin B6 S/P
Porphyrins CU Vitamin C HPG
Serotonin SG Coenzyme Q10 S/P
Vanillylmandelic acid SU

 

Key material for analysis: S=Serum, SG=Serum frozen, P=Plasma, HPG=Heparin plasma frozen, U=Urine, CU = Collected urine, E=EDTA blood

 

LC-MS/MS was used instead for all medications determined by chromatography.

 

Your contact person

Head

Dr. sc. nat. Rolf Dietiker

Special Analytics, Drug Analytics

044 269 99 59

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