(Ultra) High Performance Liquid Chromatography (HPLC/UHPLC)
The high-performance liquid chromatography (HPLC) technique involves separating the substances in a sample in a chromatography column according to their affinities to a liquid phase (eluent) and a solid phase (column material).
Using flow rates of 0.5 to 2 mL/min, pressures of up to 300 bar with UHPLC of even up to 1,300 bar, are built up in state-of-the-art systems and high-resolution columns.
The separated substances are then detected, identified according to their time of flow and quantified. During this process, a variety of detector types which measure absorption, fluorescence or electrochemical responses are used.
Our most important Routine determinations with UHPLC
|glycated haemoglobin (HbA1c)||E||Vitamin A/E||S/P|
|Homovanillylmandelic acid||CU||Vitamin B1||E|
|5-hydroxyindoleacetic acid||CU||Vitamin B2||E|
Key material for analysis: S=Serum, SG=Serum frozen, P=Plasma, HPG=Heparin plasma frozen, U=Urine, CU = Collected urine, E=EDTA blood
LC-MS/MS was used instead for all medications determined by chromatography.