High-performance liquid chromatography (HPLC)

The high-performance liquid chromatography (HPLC) technique involves separating the substances in a sample in a chromatography column according to their affinities to a liquid phase (eluent) and a solid phase (column material).

Using flow rates of 0.5 to 2 mL/min, pressures of up to 200 bar are built up in state-of-the-art systems and high-resolution columns.

The separated substances are then detected, identified according to their time of flow and quantified. During this process, a variety of detector types which measure absorption, fluorescence or electrical responses are used.

 

High-performance liquid chromatography (HPLC)

CD transferrin S Vitamin A/E S
Glycated haemoglobin (HbA1c) E Vitamin B1 E
Homovanillyl-mandelic acid SU Vitamin B2 E
5-hydroxyindoleacetic acid SU Vitamin B6 E
Catecholamines SU Vitamin B6 S
Metanephrines SU
Vanillyl-mandelic acid SU

 

Key – material for analysis S = Serum, U = Urine, CU = Collected urine, E = EDTA blood

 

LC-MS/MS was instead used for medications determined by chromatography.

 

Your contact person

Head

Dr Rolf Dietiker

Special Analytics, Drug Analytics

044 269 99 59

Diese Webseite verwendet Cookies, um bestimmte Funktionen zu ermöglichen und das Angebot zu verbessern. Indem Sie hier fortfahren, stimmen Sie der Nutzung von Cookies zu. Mehr Informationen