High-performance liquid chromatography (HPLC)

The high-performance liquid chromatography (HPLC) technique involves separating the substances in a sample in a chromatography column according to their affinities to a liquid phase (eluent) and a solid phase (column material).

Using flow rates of 0.5 to 2 mL/min, pressures of up to 200 bar are built up in state-of-the-art systems and high-resolution columns.

The separated substances are then detected, identified according to their time of flow and quantified. During this process, a variety of detector types which measure absorption, fluorescence or electrical responses are used.


High-performance liquid chromatography (HPLC)

CD transferrin S Vitamin A/E S
Glycated haemoglobin (HbA1c) E Vitamin B1 E
Homovanillyl-mandelic acid SU Vitamin B2 E
5-hydroxyindoleacetic acid SU Vitamin B6 E
Catecholamines SU Vitamin B6 S
Metanephrines SU
Vanillyl-mandelic acid SU


Key – material for analysis S = Serum, U = Urine, CU = Collected urine, E = EDTA blood


LC-MS/MS was instead used for medications determined by chromatography.


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Dr Rolf Dietiker

Special Analytics, Drug Analytics

044 269 99 59

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